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9 Minutes Read

Nitric Oxide: The Overlooked Master Regulator in Women’s Health, Hormones, and Healthy Aging



The 60-second big picture

Nitric oxide (NO) is a tiny gas with massive jobs: it keeps your arteries flexible, directs oxygen and nutrients into tissues, powers mitochondria, coordinates hormone signaling, calms inflammation, and even shapes the oral–gut microbiome. When NO falls—especially after estrogen declines in perimenopause and menopause—vascular stiffness, hot flashes, insulin resistance, sexual dysfunction, fatigue, and central weight gain become more likely. Re-building NO with nitrate-rich foods, targeted amino acids (L-citrulline/L-arginine), microbiome-friendly habits, and smart supplementation can help restore endothelial function, blood flow, and hormonal harmony.




What Nitric Oxide does (and why it matters for stroke/heart attack prevention)

NO is your body’s native vasodilator and signal carrier. It:

  • Relaxes arteries (supports healthy blood pressure, reduces platelet stickiness, improves flow).


  • Optimizes red-blood-cell oxygen delivery and nutrient perfusion to every organ—including the heart, brain, ovaries, thyroid, adrenals, and pancreas.


  • Drives mitochondrial energy production and biogenesis (more, healthier mitochondria → better fat oxidation and vigor).


  • Modulates inflammation/oxidative stress and supports immune balance.


  • Transmits neuronal signals, shapes vagal tone, and relaxes smooth muscle—key for thermoregulation and sexual function.


That’s why resilient NO signaling is linked to better vascular integrity, less endothelial dysfunction, and slower “vascular aging,” all of which reduce downstream risks tied to stroke and heart disease.




How the body makes NO: two complementary pathways

  1. Endogenous NOS pathway (L-arginine → NO): Endothelial nitric oxide synthase (eNOS) converts L-arginine to NO (needs oxygen and cofactors). Healthy eNOS = elastic vessels.


  2. Entero-salivary nitrate pathway (dietary nitrate → nitrite → NO):


    • You eat nitrate-rich plants (beets, spinach, arugula, celery, lettuce).


    • Nitrate is absorbed, circulates, and is actively concentrated in saliva by sialin.


    • Oral bacteria reduce nitrate to nitrite; when you swallow, nitrite is further reduced to NO in the stomach and in low-oxygen tissues.


    • This “recycling loop” sustains NO even when eNOS is stressed.


    • A 2021 narrative review details this pathway and why consistent dietary nitrate is a practical health strategy. BioMed Central 

Key implication: You can bypass a sluggish eNOS system (common with aging/oxidative stress) by feeding the nitrate–nitrite–NO pathway with food and microbiome-smart habits.




Why NO declines with age (and why menopause accelerates the slide)

  • Endothelial NO production drops with aging, elevating vascular stiffness and blood pressure.


  • Estrogen normally stimulates eNOS (via ERα/ERβ → PI3K/Akt), increasing NO.


  • As estrogen falls in peri/menopause: eNOS activity and NO bioavailability fall, oxidative stress rises, and eNOS can “uncouple” (producing superoxide instead of NO). Consequences: stiffer arteries, hot flashes, sexual dysfunction, low energy, higher cardiometabolic risk.


Clinical take-home: recovering antioxidant capacity + feeding the nitrate pathway + supporting eNOS can restore endothelial flexibility—a modifiable piece of cardiovascular risk.




Evidence in women: what the clinical trials show

  • Single dose (~600 mg nitrate as beetroot juice) protected the endothelium against ischemia-reperfusion injury in early postmenopausal women, improving flow-mediated dilation (FMD)—a gold-standard measure of endothelial health.


  • 7 days (~400 mg nitrate/day) clinically improved baseline FMD by ~2.2% vs placebo in postmenopausal women—meaningfully better macrovascular function within a week.


  • 12 weeks (~550 mg nitrate/day as beet extract) in women 60–85 reduced carotid-femoral pulse wave velocity and augmentation index (less arterial stiffness) with 5× higher serum nitrate and 1.5× higher nitrite—evidence of sustained NO availability.


  • A 90-day randomized, double-blind, placebo-controlled study of an inorganic nitrate complex showed ~12.5-point systolic BP reduction and ~22% FMD improvement, with nitrate/nitrite rising in plasma and saliva.


  • Pharmacokinetics: a single serving of a standardized inorganic nitrate supplement maintained elevated salivary and plasma nitrate/nitrite over 24 hours, supporting practical once- or twice-daily use.


Bottom line: Across acute (single dose), short-term (7 days), and longer-term (12 weeks to 90 days) studies, dietary nitrate improves endothelial function and/or arterial stiffness in postmenopausal women—directly countering vascular aging biology.




NO and the endocrine network (why hormones “feel” better when flow improves)

Endocrine glands are highly vascular. NO keeps their microcirculation open and coordinates local signaling:

  • Hypothalamus/Pituitary: Modulates GnRH, LH/FSH, GH, oxytocin—shaping cycles, libido, lactation, stress resilience.


  • Ovaries: Crucial for folliculogenesis, ovulation, luteal maintenance, angiogenesis, and steroidogenesis.


  • Endometrium/Uterus: Supports receptivity/implantation; dysregulated NO links to dysmenorrhea and endometriosis.


  • Adrenals: Modulates catecholamine release and HPA-axis tone.


  • Thyroid: Regulates blood flow and hormone output; dysregulated NO/iNOS activity intersects with autoimmunity & oxidative stress.


  • Pancreas & Metabolism: Orchestrates insulin/glucagon; NO imbalance contributes to insulin resistance; NO also interacts with ghrelin/leptin to influence hunger, satiety, and body composition.


Menopause link: Estrogen → eNOS → NO; when estrogen falls, vasomotor instability, sleep disturbance, endothelial dysfunction, and cognitive/mood changes increase. Rebuilding NO can help steady these systems.




Mitochondria, fat oxidation, brown fat & energy

Physiologic NO regulates mitochondrial respiration and stimulates mitochondrial biogenesis, improving energy output and beta-oxidation (fat burning). Healthier mitochondrial networks support endurance, metabolic flexibility, and lower central adiposity—key for midlife weight control.




Sexual function & autonomic tone: early clinical signals

A randomized, placebo-controlled pilot (30 days of daily nitrate) in adult women showed:

  • FSFI Desire increased +1.03 vs placebo (p=0.017);


  • FSFI Orgasm increased +2.32 vs placebo (p=0.016);


  • Wearables (e.g., Oura Ring) suggested directional improvements in cardiovascular–autonomic regulation and sleep quality, consistent with better perfusion and thermoregulation.





When NO runs low: a practical symptom “scorecard”

If multiple items below resonate, consider testing and a targeted NO-restoration plan:

  • Cold hands/feet; easy chills


  • Exercise intolerance; slow recovery


  • Brain fog; cognitive fatigue


  • Sexual dysfunction (desire, arousal, orgasm); vaginal dryness


  • Poor wound healing; easy bruising


  • Hot flashes/thermoregulatory swings


  • Central adiposity (“meno-belly”), rising A1C/glucose


  • Muscle loss; low vigor or motivation


  • Elevated BP; headaches; dizziness on standing


  • Low mood, low libido, sleep disruption


  • Peri/postmenopausal symptoms intensifying


Common root causes of NO depletion: oxidative stress, chronic inflammation, sedentary lifestyle, low-nitrate diet, gut/oral dysbiosis, environmental toxins, and age-related eNOS dysfunction.




Testing at home (and how to use the data)

Saliva NO (nitrite) strips are inexpensive, non-invasive, and give instant color-change feedback. Use:

  • First thing in the morning (baseline), before brushing/rinsing;


  • Again 2–3 hours after a nitrate-rich meal or supplement to confirm conversion;


  • Track over days/weeks; aim to keep readings in your strip’s “adequate/target” color band.
    Multiple vendors sell these strips online and in clinics; see options below.


Pro tip: Nitrate content in beets and greens varies widely by soil/season/processing—use strips to personalize your dose rather than guessing. PMC




Microbiome matters: don’t sabotage the nitrate cycle

Because oral bacteria convert nitrate → nitrite, broad-spectrum antiseptic mouthwashes can blunt the entero-salivary pathway and have been linked to higher blood pressure in controlled studies (e.g., chlorhexidine twice daily for a week raised SBP ≥5 mmHg in ~50% of subjects). Favor gentle oral care unless antiseptics are medically indicated. PMC




Food first: a women-centered, NO-rebuilding plate

High-nitrate vegetables (rotate for diversity): arugula, spinach, beets/beetroot juice, celery, lettuce (and other leafy greens). Science reviews consistently identify leafy greens and beets as top sources; exact nitrate content fluctuates across batches. BioMed CentralScienceDirect

Daily target: Based on trials in postmenopausal women, build toward ~400–600 mg nitrate/day from food on most days. Practically, that looks like:

  • Lunch: Big arugula/leafy-green bowl + roasted beets or beet hummus.


  • Smoothie: spinach/arugula + citrus + ginger; optional beet crystals.


  • Dinner: mixed greens, celery, herbs.
    Because food nitrate content varies, confirm with saliva strips and adjust. BioMed CentralPMC


Menopause-wise upgrades:

  • Add polyphenol-rich foods (berries, pomegranate, cocoa) to buffer oxidative stress that uncouples eNOS.


  • Combine nitrate-rich plates with resistance training (shear stress up-regulates eNOS) and aerobic intervals to amplify endothelial gains.





Supplements (beyond any single brand): what actually helps—and how to choose

1) Standardized dietary nitrate

  • Beetroot juice shots with guaranteed nitrate content (e.g., Beet It Sport Nitrate 400 delivers ~400 mg nitrate/70 mL per shot; used widely in research). Useful for acute boosts or daily steady state.Beet It Sport Nitrate 400

  • Red spinach (Amaranthus dubius) extracts can also supply standardized nitrate in powdered drink mixes (example product provides ~500 mg/serving).


Dose ideas, anchored to trials:

  • Acute: 400–600 mg nitrate 2–3 hours before demanding exertion or stressful events.


  • Daily: 400–600 mg/day for endothelial support; 8–12 weeks for stiffness metrics. (Use strips to tailor.)


2) Amino acids for the eNOS pathway

  • L-citrulline (often better tolerated/effective than arginine for raising plasma arginine & NO): 1.5–3 g once or twice daily. PMC+1 

  • L-arginine: 3–6 g/day, divided, can support NO synthesis, though GI tolerance varies; consider if citrulline isn’t available/appropriate. PMC 

Combining dietary nitrate + L-citrulline supports both NO pathways—useful when genetics, oxidative stress, or menopause blunt eNOS.

3) Microbiome-friendly oral care

  • Avoid routine antiseptic mouthwash unless prescribed; if needed, keep duration minimal and retest NO after you stop. PMC 

4) Reality check on non-standardized “beet” capsules

  • Many beet powders/capsules do not guarantee nitrate content—effects can be inconsistent. Prefer brands that state nitrate mg/serving or rely on test-and-adjust with saliva strips. PMC 




Where to get saliva NO test strips and standardized nitrate

Here are vetted, commonly available options (no affiliation):

  • HumanN Nitric Oxide Test Strips (25 ct; widely available online).HumanN Nitric Oxide Test Strips

  • Berkeley Life NO Test Strips (consumer and professional formats).Berkeley Life NO Tests Strips

  • Designs For Health Nitric Oxide Saliva Test Strips (50 ct) Nitric Oxide Test Strips

  • Beet It Sport Nitrate 400 (400 mg nitrate/shot; research-standardized). BEET IT Sport Shot

  • Piping Rock Beet Root Powder Capsules  (8000mg/320 ct) Piping Rock Beet Root Capsules 


400 mg shot

Beet It Sport Nitrate 400 Shot (400 mg nitrate)

$49.99

Beet It US + others






Saliva strips (25)

HumanN Nitric Oxide Indicator Strips (25 ct)

$14.00

HumanN





Saliva strips

Berkeley Life Nitric Oxide Test Strips

$9.99

Berkeley Life






50-strip tube

Nitric Oxide Saliva Test Strips (50 ct) – Designs For Health

$39.78

Designs For Health









Alt 400 mg source

Beet It Sport Nitrate 400 (alt retailer)

$43.54

Amazon





Beet capsules (non-std)

Beetroot Capsules (variable nitrate)

$52.79

Piping Rock 



Why these? They either standardize nitrate dose (making dosing predictable) or provide easy home testing so you can personalize intake. (Note: beet capsules can support general nutrition but may not deliver a reliable nitrate dose unless specified; rely on strips to verify.) PMC




A practical, female-focused, 4-week NO-restoration plan

Week 1: Baseline & Foundations

  • Test saliva NO AM/PM × 3 days; log symptoms (scorecard above).

  • Add 1–2 high-nitrate servings daily (e.g., big arugula salad + cooked spinach, or beet smoothie).

  • Ditch daily antiseptic mouthwash; gentle oral care only. PMC 

Week 2: Dose to target

  • If AM strips still low, add one standardized nitrate dose (~400–500 mg/day) OR increase greens/beets.

  • Begin L-citrulline 1.5–3 g/day (or L-arginine 3–6 g/day if preferred). PMC+1 

  • Movement prescription: 3×/week zone-2 cardio (30–45 min) + 2×/week resistance training to upregulate eNOS and improve endothelial shear stress.


Week 3: Consolidate

  • Re-test strips; adjust food/supplements to keep in target color band.

  • Layer in polyphenols (berries, pomegranate) + sleep hygiene to curb oxidative stress/uncoupling.


Week 4: Personalize

  • If vasomotor symptoms, sexual dysfunction, or exercise intolerance persist, consider maintaining daily nitrate + citrulline, then reassess at 8–12 weeks (the timeframe used in arterial stiffness trials).





Labs your clinician can order (to connect dots)

  • Endothelial/vascular: FMD (research clinics), pulse-wave velocity/augmentation index, resting BP & HRV trends.

  • Metabolic: fasting glucose, A1C, fasting insulin ± HOMA-IR, lipids, hs-CRP.

  • Thyroid: TSH, Free T3/T4, TPO/Tg antibodies (if autoimmune risk).

  • Sex hormones: E2, progesterone, LH/FSH, SHBG ± total/free T; consider day-specific sampling.

  • Other context markers: ferritin, B12/folate, vitamin D, magnesium, homocysteine (for redox status).

  • Optional genetics (context): polymorphisms affecting eNOS or redox balance may modulate NO bioavailability, but interventions (dietary nitrate + eNOS support + microbiome care) remain first-line.





Safety & smart use

  • Dietary nitrate in vegetables is safe for most adults; it is not the same as nitroglycerin (prescription) or nitrite preservatives.

  • If you’re on antihypertensives, PDE-5 inhibitors, or have kidney disease, low BP, or bleeding disorders, coordinate with your clinician before adding concentrated nitrate or high-dose amino acids.

  • If you must use antiseptic mouthwash (gum disease, post-procedure), keep it short and re-check strips when you stop. PMC 




Why this is especially relevant to midlife women

Estrogen stimulates eNOS and helps you synthesize and use NO; the menopausal drop is a perfect storm for vasomotor instability, endothelial dysfunction, sleep fragmentation, and metabolic drift. Re-building NO—with food, targeted nitrate, citrulline, and microbiome-friendly habits—directly addresses these mechanisms and has now shown clinical improvements in endothelial function and arterial stiffness in postmenopausal women.




Citations from your slides (selected)

Mechanisms, menopause biology, entero-salivary pathway, endocrine links, mitochondria, and women-specific clinical trials are detailed throughout your uploaded decks.

Additional supporting sources

  • High-nitrate vegetables & population health review; variability in beet products. BioMed CentralPMC

  • Mouthwash can blunt nitrate→nitrite conversion and raise BP in controlled settings. PMC 

  • L-citrulline vs L-arginine physiology and dosing. PMC+1

  • Standardized nitrate products with guaranteed mg/serving. BEET IT Sport Shot 




Final take-home

NO is not just a “blood-vessel molecule.” It’s a master integrator across brain, metabolic, gut, cardiovascular, reproductive, and mitochondrial systems—a true vitality biomarker for women. Build it with nitrate-rich plates, eNOS pathway support (citrulline/arginine), microbiome-friendly oral care, and objective home testing. Then let your symptoms, strip readings, and wearable trends confirm the changes.

If you’d like, I can tailor this into:

  • a shorter, SEO-ready post with skimmable takeaways,


  • a clinic handout (with the symptom scorecard and a 1-page protocol), or


  • a patient-friendly “NO-boosting meal map” with precise recipes and prep steps.



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Mediterranean-style eating (more whole foods, olive oil, fish) reduces inflammation.Type 2 Diabetes: More UPFs = higher risk of insulin resistance and diabetes.Dementia: Studies show higher UPF intake increases dementia risk, while whole-food diets lower it.Heart Disease & Autoimmune Issues: Chronic inflammation from diet plays a huge role in both.What You Can Do This WeekMy “Calm the Fire” BasicsCrowd in whole foods. Build meals around protein + colorful plants + healthy fats (like olive oil, nuts, avocado).Swap UPFs for upgrades:Soda → sparkling water + splash of juicePackaged cookies → apple slices + almond butterProcessed deli meats → roasted chicken or beansWhite crackers → whole grain optionsCheck labels. If you see CMC or polysorbate-80, put it back.Cook wetter. Stew, steam, or braise more often to lower AGEs.Fiber is your friend. Aim for 25–35g/day from plants and whole grains.Copy and Printable: Your Quick GuideUPF Swap ListSoda → Sparkling water + fruitChips → Air-popped popcornPackaged sweets → Fruit + nutsProcessed meats → Lentils, beans, roasted poultryWhite bread → Whole grain sprouted bread or gluten free breadAdditives to WatchCarboxymethylcellulose (CMC)Polysorbate-80High-fructose corn syrupArtificial colors/flavors👉 Download this printable card here Final ThoughtsYou don’t have to be perfect. Functional medicine isn’t about restriction—it’s about making swaps that give your body what it needs to cool the fire. If you start by reducing UPFs and focusing on colorful, whole foods, you’ll see inflammation markers drop, energy rise, and long-term risk for arthritis, diabetes, and dementia shrink.And remember—every small choice counts.Call to ActionIf you’re ready to learn more about how to lower inflammation with food, subscribe to my podcast at Larson Health Podcast or check out my latest recipe Pesto & Hummus Turkey Roll Ups. By Kim Larson — Larson HealthLet’s Talk Real for a MinuteHey friend, Kim here 👋. Let’s have an honest talk about something that affects almost all of us: highly processed foods. These are the foods that come in flashy packages, usually with long ingredient lists, and they’re so common in today’s diet that we barely notice them anymore.The problem? They keep our bodies in a state of chronic, low-grade inflammation—and that “fire inside” can set the stage for conditions like arthritis, diabetes, dementia, heart disease, and even autoimmune flares.Don’t worry, I’m not here to guilt-trip you out of ever eating chips again. I’m here to give you the facts, in plain English, and show you simple swaps you can make this week that calm inflammation and help you feel like you again.What Are “Highly Processed Foods”?Researchers call them ultra-processed foods (UPFs). These aren’t just frozen veggies or plain canned beans (those are fine, by the way!). UPFs are foods that are:Built from refined starches/sugars, industrial oils, and additivesLow in fiber, vitamins, and mineralsDesigned to be hyper-palatable (you know, the “can’t eat just one” effect)Think: sodas, packaged pastries, sweetened yogurts, chips, energy bars, fast food, frozen pizza, flavored cereals, and many packaged snacks.Studies link high UPF intake to more inflammatory markers in the blood and higher risk of chronic disease.Why Do Processed Foods Stoke Inflammation?1. Blood Sugar RollercoasterAll that refined sugar and white flour sends your blood sugar spiking, then crashing. Over time, this rollercoaster pushes your immune system into a “low simmer” of inflammation.➡️ Want to see how I help balance blood sugar with food? Check out my recipe for Steak Bites & Mashed Sweet Potatoes.2. Gut Irritants from AdditivesSome common food additives like carboxymethylcellulose (CMC) and polysorbate-80 (often in packaged ice creams, dressings, and sauces) have been shown to thin the gut lining and irritate the microbiome. When the gut lining is irritated, the immune system turns on.3. Advanced Glycation End Products (AGEs)When foods are cooked at high, dry heat (like fried fast food), they form compounds called AGEs. Too many AGEs = more oxidative stress and inflammation in your body.4. Damaged Oils from Deep FryingIt’s not just the oil—it’s the process. Reheated oils (like in fast food fryers) break down into compounds that are toxic to our cells.➡️ Listen to my podcast episode “Fasting Hacks You Need to Know About” where I talk more about how food quality matters during fasting.Diseases Linked to InflammationArthritis: Western, processed diets are linked with more joint pain and swelling. Mediterranean-style eating (more whole foods, olive oil, fish) reduces inflammation.Type 2 Diabetes: More UPFs = higher risk of insulin resistance and diabetes.Dementia: Studies show higher UPF intake increases dementia risk, while whole-food diets lower it.Heart Disease & Autoimmune Issues: Chronic inflammation from diet plays a huge role in both.What You Can Do This WeekMy “Calm the Fire” BasicsCrowd in whole foods. Build meals around protein + colorful plants + healthy fats (like olive oil, nuts, avocado).Swap UPFs for upgrades:Soda → sparkling water + splash of juicePackaged cookies → apple slices + almond butterProcessed deli meats → roasted chicken or beansWhite crackers → whole grain optionsCheck labels. If you see CMC or polysorbate-80, put it back.Cook wetter. Stew, steam, or braise more often to lower AGEs.Fiber is your friend. Aim for 25–35g/day from plants and whole grains.Copy and Printable: Your Quick GuideUPF Swap ListSoda → Sparkling water + fruitChips → Air-popped popcornPackaged sweets → Fruit + nutsProcessed meats → Lentils, beans, roasted poultryWhite bread → Whole grain sprouted bread or gluten free breadAdditives to WatchCarboxymethylcellulose (CMC)Polysorbate-80High-fructose corn syrupArtificial colors/flavors👉 Download this printable card here Final ThoughtsYou don’t have to be perfect. Functional medicine isn’t about restriction—it’s about making swaps that give your body what it needs to cool the fire. If you start by reducing UPFs and focusing on colorful, whole foods, you’ll see inflammation markers drop, energy rise, and long-term risk for arthritis, diabetes, and dementia shrink.And remember—every small choice counts.Call to ActionIf you’re ready to learn more about how to lower inflammation with food, subscribe to my podcast at Larson Health Podcast or check out my latest recipe Pesto & Hummus Turkey Roll Ups.

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